Fig. 2: Condition optimisation for PuuE tube assembly.
From: Protein design of two-component tubular assemblies similar to cytoskeletons

a, b The kinetics of tubular assembly. nsTEM images of tubular assembly (a) and length analysis (b). c nsTEM images of tubular assemblies with varying NaCl concentration. d nsTEM images showing the reversibility of tube structures with changing NaCl concentration. e Tube length analysis of nsTEM images. For tube length analysis, tubes were picked up and calculated from 5k images at each step; 150 tubes from the longest tube length were used at each data point. *** p < 0.001 (p-values; 1st–2nd = 9.98 × 10–4 and 2nd–3rd = 3.63 × 10–6, two-sided Welch’s t test). Scale bar, 1 µm. Source data are provided as a Source Data file.