Fig. 2: A distal cis-regulatory module (CRM) and the core promoter that are necessary and sufficient for bundle sheath expression.
From: A transcription factor ensemble orchestrates bundle sheath expression in rice
a Schematics showing deletions of nucleotides −980 to −829 and −251 to +42. b Representative image of leaf cross sections of transgenic lines after GUS staining. Zoomed-in images of lateral veins shown in right panels, the staining duration is displayed in the bottom-left corner, bundle sheath cells highlighted with dashed red line, scale bars = 50 µm. c Promoter activity determined by the fluorometric 4-methylumbelliferyl-β-D-glucuronide (MUG) assay. Data subjected to pairwise two-sided Wilcoxon rank-sum test with Benjamini-Hochberg correction for multiple comparison. Lines with differences in activity that were statistically significant (adjusted P < 0.05) were labelled with different letters. The median represents the median GUS activity value and is shown as red line in the plot, n indicates the number of T0 transgenic plants analysed. Source data are provided as a Source Data file.
