Fig. 5: Oligomerisation of bundle sheath cis-regulatory module (CRM) increases bundle sheath expression.
From: A transcription factor ensemble orchestrates bundle sheath expression in rice
a, d Schematics showing site-directed mutagenesis of WRKY, G2-like, MYBR, IDD and bZIP motifs, mutated nucleotides highlighted in red (a), and constructs to test the impact of oligomerization of the CRM (d). b, e Representative images of cross sections from transgenic lines after GUS staining, zoomed-in images of lateral veins shown in right panel, the staining duration is displayed in the bottom-left corner, bundle sheath cells highlighted with red dashed lines, scale bars = 50 µm. c, f Promoter activity determined by the fluorometric 4-methylumbelliferyl-β-D-glucuronide (MUG) assay. Data subjected to pairwise two-sided Wilcoxon rank-sum test with Benjamini-Hochberg correction. Lines with differences in activity that were statistically significant (adjusted P < 0.05) were labelled with different letters. Median catalytic rate of GUS indicated with red lines, n indicates total number of T0 transgenic plants assessed. Source data are provided as a Source Data file. g Paradermal view of Arabidopsis leaf expressing GUS under the control of 3x BS CRM combined with OsSiR core promoter, the staining duration is displayed in the bottom-left corner. M indicate mesophyll, BS for bundle sheath, and V for vein. Zoomed in images for the red box shown on right, bundle sheath highlighted with red dash lines.
