Fig. 2: Host-guest dependent ion transport activities determination.
a Mixing h, H, t-3 and c-3 generates only host-guest complex H•t-3. b The LUV-based pH-sensitive HPTS assay for evaluating ion transport activities of 3 at 2.0 μM; here, RK+ = (IK+ – I 0)/(I Triton − I 0) whereas I K+ and I0 are the ratiometric values of I 460/I 403 at t = 300 s before addition of triton, and I Triton is the ratiometric value of I 460/I 403 at t = 300 s right after addition of triton. c The ion transport activities of t-n (n = 1–3) in the presence of host h. d The ion transport activities of t-n (n = 1–3) in the presence of host H. HPTS = 8-Hydroxypyrene-1,3,6-trisulfonic acid trisodium salt. All data represent mean ± SD from three independent experiments (n = 3).
