Fig. 3: DNMT1 regulates the migration of SST⁺ interneurons in the developing cortex.

a Scheme of cIN migration routes (E14.5, coronal section, hemisphere). b, c Sst-Cre/tdTomato (Ctrl; b) and Sst-Cre/tdTomato/Dnmt1 loxP² (KO; c) brain sections (50 µm). Scale bars: 500 µm. d TdTomato⁺ cell distribution in basal telencephalon vs. cortex (E14.5; Ctrl: n = 12 sections, KO: n = 8 sections, N = 3 embryos/genotype). e TdTomato⁺ cell count within the cortex normalized to the area (50 µm, Ctrl: n = 14 sections, KO: n = 8 sections, N = 3 embryos per genotype). f Migration trajectories within the E14.5 cortex. g, h Ctrl and KO cortices (E14.5); white asterisks: cells in MZ or IZ/SVZ; yellow arrows: cells in CP; scale bars: 100 µm. i Proportional distribution of tdTomato⁺ cells within the cortical zones normalized to the overall tdTomato⁺ cell count (E14.5, Ctrl: n = 14 sections, KO: n = 8 sections, N = 3 embryos per genotype). j, k Temporal z-projections of tdTomato⁺ cell migration over 20 h (350 µm, Ctrl vs. KO). Scale bars: 500 µm. l–o Quantifications: l tdTomato⁺ fluorescence in CP (first 12 h; Ctrl: n = 4, KO: n = 6 slices, N = 3 embryos/genotype); m average directional changes (Ctrl: 134 cells/5 slices, N = 4 embryos; KO: 74 cells/3 slices, N = 3 embryos); n velocity of all migrating cells (Ctrl: 377 cells/6 slices, N = 5 embryos; KO: 323 cells/5 slices, N = 3 embryos); o velocity in CP–VZ (Ctrl: 193 cells/6 slices, N = 5 embryos; KO: 114 cells/3 slices, N = 3 embryos). p, q Migration of tdTomato⁺ cells (circled, 350 µm slices, Ctx; scale: 50 µm). *p < 0.05, **p < 0.01, ***p < 0.001; n.s.: not significant (further information: Supplementary Data 10). Ctrl control, CP cortical plate, VZ ventricular zone, SVZ subventricular zone, MZ marginal zone, IZ intermediate zone, Ctx cortex. Data points sharing the same symbol in (e) and (m–o) represent one embryo. Nested two-way ANOVA in (d), (e), (i), (l–o). Error bars: ±SEM.