Fig. 7: Aberrant cortical architecture and functionality in Dnmt1-deficient mice. | Nature Communications

Fig. 7: Aberrant cortical architecture and functionality in Dnmt1-deficient mice.

From: DNMT1-mediated regulation of somatostatin-positive interneuron migration impacts cortical architecture and function

Fig. 7

a, b CUX1 immunostainings with DAPI (six-month-old male Sst-Cre/tdTomato/Dnmt1 loxP2 (KO) and Sst-Cre/tdTomato mice (Ctrl), respectively). S1 sagittal slices from Bregma 1.32 and 1.44. Scale bars: 100 µm. c, d tdTomato+ cell density normalized to cortical area (c), cortical thickness, and proportional thickness of deep and upper layers (d; nested two-way ANOVA; Ctrl: n = 24 slices, KO: n = 23 for, N = 3 brains per genotype. *p < 0.05 (detailed information: Supplementary Table 10). Error bars: ±SEM from the mean. Data points with same symbols depict the same mouse. e S1 Neuropixels recordings in head-fixed mice on a wheel. Example brain slice: probe fluorescence and schematic position. Scale bar: 200 µm. f Average CSD across depth, following a brief tactile stimulus (white line). Blue and yellow colors indicate current sinks and sources. g Significant lower and delayed (above 400 µm) CSD response peaks across sessions in Dnmt1 KO mice (peak amplitudecontrol = −4.74 ± 1.22 µA/mm3, peak amplitudeKO = −1.41 ± 0.18 µA/mm3, p = 0. 0037; peak timecontrol = 27.20 ± 0.84 ms, peak timeKO = 31.80 ± 1.03 ms, p = 0.0041; n = 8 sessions). h Average CSD traces across tactile responses in supragranular (top) and infragranular layers (bottom). i Peristimulus time histogram for the spiking response to tactile stimulation in supra- or infragranular S1 layers. j LFP spectral power density (PSD) across depth in S1 (left), V1 (right; S1: gamma powercontrol = −23.69 ± 21.32 dBmV, gamma powerKO = −62.22 ± 3.16 dBmV, p = 0.0201; V1: gamma powercontrol = −25.65 ± 8.65 dBmV, gamma powerKO = −66.70 ± 4.72 dBmV, p = 0.0069; n = 8 sessions). k Stimulus-induced PSD change in V1, relative to baseline (gammacontrol = 104.25 ± 24.42%, gammaKO = 27.75 ± 7.20%, p = 0.0069; n = 8 sessions). l Stimulus-induced PSD change across depth. g, j, k: Wilcoxon rank-sum test, mean ± SEM. Shading in (h–k) shows the ±SEM. 2 mice per genotype in (g–k). Raw data available via hyperlinks in the Data Availability Statement.

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