Fig. 1: Immune infiltration dynamics during breast cancer progression.
From: Mogat1 drives metabolic adaptations to evade immune surveillance
a Schematic diagram of the orthotopic breast cancer model utilizing MMTV-PyMT tumor cells inoculated into fat pad of immunocompetent FVB mice. Created in BioRender. https://BioRender.com/penf9v4. b Tumor growth kinetics of MMTV-PyMT tumors in FVB mice (n = 5 mice per group). c–e Flow cytometry analysis of total immune cell infiltration in MMTV-PyMT tumors at different time points. c Percentage of CD45+ immune cells among live cells within the tumor. d Total number of CD45+ immune cells isolated from per gram tumors. e Representative flow cytometry plots CD45+ immune cells gated on live cells. (n = 5 mice per group). f Representative immunofluorescence images of MMTV-PyMT tumor sections at indicated time points, stained for CD45 (green) to visualize total immune cells. Scale bar: 100 μm. g Quantification of CD45+ immune cell density (cells/mm2) in MMTV-PyMT tumors. (Data represent the mean value from n = 5 biologically independent mice per group. For each mouse, 3 tumor sections were analyzed, and the average value was used for plotting and statistical analysis.). h Quantification of the distance between CD45+ cells and the tumor margin in MMTV-PyMT tumors, assessed from immunofluorescence images. (Data represent the mean value from n = 5 biologically independent mice per group.). i, j Flow cytometry analysis of T cell and NK cells within the tumor-infiltrating lymphocyte population at different time points. i Representative flow cytometry plots gated on lymphoid cells (gated CD45+ CD11B- cells). j Quantification of T cells (CD3+ NK1.1-) as a percentage of lymphoid cells. The gating strategy for these panels is further detailed in Supplementary Fig 2a. (n = 5 mice per group). Data c–j are representative of two independent experiments from 5 mice per group per experiment. Data are presented as mean ± SD (d, e, g, h, j) and mean ± SEM (b). Statistical significance was calculated using one-way ANOVA (d, e, g, h, j). Source data are provided as a Source Data file.
