Fig. 7: Mogat1 loss in tumor cells stimulate anti-tumor immunity by T cells in vivo.

a Schema of CD4/8 antibody depletion in orthotopic MMTV-PyMT breast tumor-bearing immunocompetent FVB mice. b Frequency of CD8⁺ and CD4⁺ T cells within tumor after inject IgG or CD4/8 antibody with flow cytometry in day 26. See Supplementary Fig. 8a, b for gating strategy. c Tumor growth of FVB immunocompetent mice inoculated with shCTRL and shMogat1 MMTV-PyMT tumor cells versus T cell depletion. (n = 6-8 mice per group). d, e Tumor volumes and tumor weight from (c) at day 26. (n = 8 mice in anti-IgG group; In anti-CD4/8 group, n = 7 mice in shCTRL and shMogat1_2 group, n = 6 mice in shMogat1_1 group). f Schematic representation of MDA-MB231 orthotopic tumor model in severe immunodeficient NOG mice. g Tumor growth of severe immunodeficient NOG mice inoculated with MDA-MB231 tumor cells. h Tumor volumes and tumor weight at day 34. (n = 7 mice in shCTRL and shMogat1 group, n = 6 mice in shMogat1+ OE group). i Schematic representation of MDA-MB231 orthotopic tumor model in MHCI/II-DKO NOG mice tail vein injected human PBMC at day 0 and day 16. (n = 6–7 mice per group). j Tumor growth of MHCI/II-DKO NOG mice inoculated with MDA-MB231 tumor cells. k Tumor volumes and tumor weight at day 36. (n = 6 mice in shCTRL and shMogat1 group, n = 7 mice in shMogat1+ OE group). l The proportion of tumor-infiltrating human CD8⁺ T cells was detected by flow cytometry in MHCI/II-DKO NOG mice at day 36. Gated mCD45⁺hCD45⁺ cells. (n = 5 mice per group). The flow cytometry gating strategy is presented in Supplementary Fig. 9c, d. m Schema of B16/F10 melanoma animal model in Rag1^-/- mice. (n = 5 mice per group). n Growth curves of B16/F10 tumors in immunodeficient Rag1^-/- mice. o Quantification of tumor volume at day 15 in the B16/F10 model. (n = 5 mice per group). Data are representative of two independent experiments. Data are presented as mean ± SD (d, e, h, k, l, o) and mean ± SEM (c, g, j, n). Statistical analysis was conducted by two-way ANOVA followed by Tukey’s multiple comparison test (c, g, j, n). Statistical significance was calculated using unpaired Student’s t tests (o) and one-way ANOVA (d, e, h, k, l). ns, not significant. Figure (a, f, i, m) are created in BioRender. https://BioRender.com/yduci19. Source data are provided as a Source Data file.