Fig. 5: Transcriptional changes of immune cells in the gut in a mouse model of typhoid fever.
From: Salmonella Typhi gut invasion drives hypoxic immune subsets associated with disease outcomes
a Overview of the experiment: mice were orally infected with S. Typhimurium, without streptomycin pretreatment. Immune enriched samples were collected from ileum, cecum, spleen, and PP before infection and 24 or 72 h post-infection (hpi) and analyzed by bulk RNA-seq. b–e Principal component analysis (PCA) projection of the bulk RNA-seq data based on all expressed genes onto the space of the two leading principal components for all samples together (b), ileum and cecum samples alone (c), spleen samples (d), and PP samples (e), showing tissue-specific separation independent of infection, and infection-induced separation within the spleen and most pronounced in the PP. Color coding for tissues and time points corresponds to (a). f Gene Set Enrichment Analysis (GSEA) comparing S. Typhimurium infected PP at 72 hpi versus naïve PP identifies up-regulated (right) and down-regulated (left) immune and metabolic pathways in infected PP. g Heatmaps of the top significant pathways from (f), showing the expression levels of the corresponding genes in naïve versus infected PP. The color bar indicates relative expression levels, and representative genes for each pathway are highlighted. Created in BioRender. Hen-avivi, S. (2025) https://biorender.com/dt9zwpe.
