Fig. 4: Experimental results of BMS-262084 inhibition. | Nature Communications

Fig. 4: Experimental results of BMS-262084 inhibition.

From: Simulations and active learning enable efficient identification of an experimentally-validated broad coronavirus inhibitor

Fig. 4

a Dose-response curves and IC50 estimates for inhibition in TMPRSS2 biochemical assay. For BMS-262084, the average (mean) ±SD of three technical replicates is shown. b IC50 as a function of pre-incubation time, estimates of IC50 at infinite time and time at which the function reaches its minimum. c, d Inhibition of live SARS-CoV-2 infection of Calu-3 cells. PFU, plaque-forming units. The average (mean) ±SD of three technical replicates is shown. Statistical significance was analyzed by two-way analysis of variance (ANOVA) with Dunnetts post hoc test. P values (for concentrations between 5 and 50,000 nM, from left to right) are as follows: AY.1 (0.9911, 0.9992, <0.0001, 0.0031, <0.0001, <0.0001, <0.0001, <0.0001, <0.0001, <0.0001) and KP.3.1.1 (0.9995, 1.0000, <0.0001, <0.0001, <0.0001, <0.0001, <0.0001, <0.0001, <0.0001, <0.0001). e–l Dose-response curves and IC50 estimates for inhibition of pseudovirus cell entry into Calu-3 cells driven by VSV-G (control, dashed lines) or S protein (solid lines) of SARS-CoV-2 lineages B.1, B.1.617.2, EG.5.1 and BA.2.86 or coronaviruses HCoV-NL63, HCoV-229E, SARS-CoV-1 and MERS-CoV, respectively. The average (mean) ±SD of three biological replicates is shown. Each biological replicate was performed with four technical replicates.

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