Table 1 Summary of cryo-EM samples

From: Amyloid fibril structures link CHCHD10 and CHCHD2 to neurodegeneration

Sample

Concentration (µM)

Shaking speed (rpm)

Temp (°C)

Time point (hrs)

Polymorphs with twista

Solved structures

D10-NT sample-1 (polymorph1/3)

80

1200

37

120

2

2

D10-NT sample-2 (polymorph-2)

80

1200

37

120

1

1

D2-NT

80

1200

37

192

2

1

S59L D10-NT

80

900

37

22

2

1

T61I D2-NT

80

1000

37

24

1

1

R15L D10-NT

80

1000

37

2

1

1

  1. aThe number of polymorph classes featuring twisted fibrils was assessed using the FilamentTools procedure in RELION 5.0b.
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