Fig. 4: Inter-subunit interfaces between KAP-1ΔN and the two motor tails. | Nature Communications

Fig. 4: Inter-subunit interfaces between KAP-1ΔN and the two motor tails.

From: A mutual co-recognition mechanism ensures the proper assembly of heterotrimeric kinesin-2 for intraflagellar transport

Fig. 4

a A combined surface and ribbon model showing the inter-domain interfaces that can be divided into two sites, the ARM-mediated site (interface I) and the CTH-hook-mediated site (interface II). b–d A combined ribbon and stick model showing the ARM-mediated interface I. Interface I is divided into three sub-categories (interface Ia (b), interface Ib (c) and interfaceIc (d) based on different ARM repeats from the N-terminus to the C-terminus). The sidechains of the key residues involved in the inter-domain interface packing are shown as sticks. e A combined ribbon and stick model showing the CTH-hook-mediated site (interface II). f, g Co-immunoprecipitation assay of KAP-1, KLP-20 and KLP-11 in Sf9 cells that are co-transfected with wild-type KAP-1-GFP-Strep, KLP-20-Myc and KLP-11-Flag and their various mutants. The modified pFastbac1 vector expressing the GFP-Strep tag is used as the negative control. Each experiment is independently repeated three times (n = 3). Source data are provided as a Source Data file.

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