Fig. 3: Convergent synthesis of DIGIT libraries and characterization of DIGIT/mRNA complexes.
From: Discrete Immolative Guanidinium Transporters deliver mRNA to specific organs and red blood cells
a Convergent synthesis of DIGITs from lipid alcohols. b Chemical structure of lipid substituents and the resulting DIGIT libraries evaluated in this study. yGx = DIGIT structure attached with lipid #y on the guanidinium side (orange) and with lipid #x on the ester side (purple). c Scheme to evaluate DIGIT/mRNA complexes and their degradation (surface charge and RNA encapsulation). Created with ChemDraw. d Surface charge of DIGIT/mRNA complexes over time incubated in buffers with different pH (N = 3). Data are presented as mean values +/− SD. e Evaluation of mRNA encapsulation and release by Ribogreen assay (N = 3). Data are presented as mean values +/− SD. Mann Whitney test was applied, P-value > 0.999. ns = not significant. Source data are provided as a Source Data file.
