Fig. 5: In vivo analysis of blood-specific mRNA delivery using DIGIT complexes.
From: Discrete Immolative Guanidinium Transporters deliver mRNA to specific organs and red blood cells
a Optimization of leading blood-tropic DIGIT formulation (N = 2). Data are presented as mean values +/− SD. b Evaluation of luciferase signal in blood (N = 3). Data are presented as mean values +/− SD. Ordinary one-way ANOVA was applied for multiple comparison test. P value = 0.999. ns = not significant. Icons (top) created in BioRender. Zhang, R. (2025) https://BioRender.com/c7fqb80. c Calculated selectivity of blood transfection from isolated organs (N = 3). Data are presented as mean values +/− SD. Ordinary one-way ANOVA was applied for multiple comparison test. Blood signal (Blood) was estimated by multiplying signal on plate reader by 32-fold (1600 ul blood for animal/50 ul). d Luciferase signal in different murine blood components after administration of 13G10/mfluc complex in vivo (N = 3). Data are presented as mean values +/− SD. Mann Whitney test was applied. P-value = 0.2000, ns = not significant. e Transfection of reticulocytes after administration of 13G10/eGFP mRNA complex in vivo (N = 3). Source data are provided as a Source Data file.
