Fig. 5: Growth defect of E. coli ∆IF2-Domain-I strain.

a Doubling time of WT and ∆IF2-Domain-I strains in LB and M9 media supplemented with glucose or succinate as a carbon source. Bars and error bars represent mean and standard deviation, respectively, calculated from 4 independent biological replicates, where each individually measured doubling time is shown as a circle. Statistical significance between groups was assessed using a two-sided unpaired t-test. P-values are indicated in the figure as follows: P < 0.05 (*), P < 0.001 (***). The p-values comparing WT and ∆IF2-Domain-I strains grown in LB, M9 supplemented with glucose, and M9 supplemented with succinate are 0.0009, 0.0335, and 0.0002, respectively. b, c Growth curves of WT and ∆IF2-Domain-I in M9 media supplemented with glucose (b) or succinate (c) after growing for 24 h either in LB (“unadapted” strains) or in the M9 media (“adapted” strains). All strains were diluted in fresh media to the same starting optical density. Mean growth curves for each strain and condition are shown as solid lines, individual biological replicas are shown as dashed lines, and calculated standard deviations are shown as a shadow. Source data are provided as a Source Data file.