Fig. 7: Predicting neoantigens from CTCs for tumor treatment. | Nature Communications

Fig. 7: Predicting neoantigens from CTCs for tumor treatment.

From: Identification of multiple genomic alterations and prediction of neoantigens from circulating tumor cells at the single-cell level

Fig. 7

a Workflow for predicting neoantigens. bThe number of predicted neoantigens in CTCs from KPC0402 and KPC0403 mice, and their shared neoantigens. c, d Percentages of IFNγ-secreting CD8⁺ T cells among total CD8⁺ T cells after co-culture with BMDCs followed by peptide stimulation. Data are presented as mean ± s.d. from n = 4 independent biological replicates, and analyzed using an unpaired two-tailed Student’s t test. Exact P values are shown in the figure. P values less than the detection limit (0.0001) are indicated as P < 0.0001. e Schematic of the experimental design for mouse immune experiments, with “qd.” indicating drug administration every 24 h. Tumor volume (f) and tumor weight (g) of subcutaneous tumors derived from KPC0402 and KPC0403 primary tumor cells in mice treated with neoantigen peptides. n = 7 mice per group (independent biological replicates). Data are presented as mean ± s.d., and analyzed using Tukey’s one-way ANOVA. P values < 0.05 were considered statistically significant. Exact P values are shown in the figure. P values less than the detection limit (0.0001) are indicated as P < 0.0001. Source data are provided as a Source Data file.

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