Fig. 1: A complementation-based assay to visualize ternary protein interactions. | Nature Communications

Fig. 1: A complementation-based assay to visualize ternary protein interactions.

From: Design and evaluation of a tripartite chemogenetic fluorescent reporter for visualizing ternary protein complexes

Fig. 1

a The interaction of three proteins is visualized through proximity-induced complementation of three complementary fragments of the chemogenetic fluorescent reporter pFAST. b Design of tripartite-split-pFAST through protein splitting at sites 98–99 and 114–115. The model of pFAST was generated in ref. 27. c Structures of the fluorogens 4-hydroxy-3-methylbenzylidene rhodanine (HMBR) and 4-hydroxy-2,5-dimethylbenzylidene rhodanine (HBR-2,5DM) used in this study.

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