Fig. 2: Circular permutations of FAST. | Nature Communications

Fig. 2: Circular permutations of FAST.

From: Design and evaluation of a tripartite chemogenetic fluorescent reporter for visualizing ternary protein complexes

Fig. 2

a Sequences and properties of the different circular permutations of FAST screened during this study (see also Supplementary Fig. 2 for details). b Influence of the linker on the properties of cpFAST(24–25) and cpFAST(98–99). Are reported the thermodynamic dissociation constants and the fluorescence quantum yields (FQY) of the different cpFAST::HMBR assemblies (see also Supplementary Figs. 3 and 4). Previously reported data for cpFAST(114–115) are shown for comparison.

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