Fig. 7: Visualization of the Fos·Jun·DNA ternary complex. | Nature Communications

Fig. 7: Visualization of the Fos·Jun·DNA ternary complex.

From: Design and evaluation of a tripartite chemogenetic fluorescent reporter for visualizing ternary protein complexes

Fig. 7

a Constructs for the expression of bJun-pFAST1-98, bFos-pFAST115-125 and H2B-pFAST99-114 for probing the Fos·Jun·DNA ternary complex formation. b Representative confocal micrographs of HBR-2,5DM-treated HeLa cells co-expressing bJun-pFAST1-98, H2B-pFAST99-114 and either bFos-pFAST115-125 or bFosΔ(179-193)-pFAST115-125. Cells were treated with 10 μM HBR-2,5DM. Identical imaging settings were used for direct comparison. Scale bars 20 μm. Representative confocal micrographs of n > 100 cells from three independent experiments. c Relative nuclear tripartite-split-FAST fluorescence computed by normalizing the nuclear fluorescence intensity of tripartite-split-FAST IF(tripartite-split-FAST) with the fluorescence intensity IF(iRFP670) of co-expressed iRFP670. Each cell is color-coded according to the biological replicate it came from. The solid circles correspond to the mean of each biological replicate. The black line represents the mean ± SD of the three biological replicates. Source data are provided as a Source Data file.

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