Fig. 4: MEANING induces ROS production and cell death in vitro.
From: Mitochondrial inflexibility ignites tumor immunogenicity in postoperative glioblastoma
a Cellular uptake of B-TTPy loaded in different nanoformulation by QL01#GBM cells was analyzed by flow cytometry. b Statistical analysis of the fluorescence intensity of intracellular B-TTPy at different time points. Data are shown as the mean ± S.D. (n = 5 independent experiments). c CHEK1 expression levels in QL01#GBM cells exposed to different treatments. Data are shown as the mean ± S.D. (n = 3 independent experiments). Data are analyzed with one-way ANOVA. d Statistical analysis of ROS level of QL01#GBM cells after treatment with different nanomedicines. Data are shown as the mean ± S.D. (n = 3 independent experiments) (exact P value: P = 7.10603E-07). Data are analyzed with one-way ANOVA. e Confocal laser scanning microscopy imaging of the intracellular ROS levels (green fluorescence from dichlorofluorescein (DCF) in the reaction between DCFH-DA and ROS of QL01#GBM cells following various treatments. Scale bar, 20 μm. f Cell viability was determined by CCK8 assays after treatment with each nanoformulation. Data are shown as the mean ± S.D. (n = 3 independent experiments) (exact P value: P = 4.10338E-06). Data are analyzed with one-way ANOVA. g Quantitative analysis and h Confocal live/dead cell fluorescence imaging of QL01#GBM cells treated with each formulation. Green from Calcein indicates live cells, red from PI denotes dead cells, and the yellow color results from co-staining of Calcein and PI. Data are shown as the mean ± S.D. (n = 3 independent experiments) (exact P value: B-TTPy vs. BD P = 4.46967E-06; CBD vs. MEANING P = 2.73496E-07). Data are analyzed with one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Source data are provided as a Source Data file.
