Fig. 5: MEANING caused mitochondrial damage and induced immunogenic death in malignant GBM cells.
From: Mitochondrial inflexibility ignites tumor immunogenicity in postoperative glioblastoma
a Schematic illustration of the mechanism that MEANING induce mitochondria damage. i) si-RNA silence expression of CHEK1. ii) B-TTPy molecules locate in mitochondria and produce ROS upon the light irritation. iii) mitochondria contents release and induce GBM cell immunogenicity death. b TEM image of normal mitochondria (above) and damaged mitochondria (below). Scale bar, 1 μm. c Confocal laser scanning microscopy imaging and d quantitative analysis of red fluorescence intensity from tetramethylrhodamine, ethyl ester (TMRE) in QL01#GBM cells. Scale bar, 15 μm. Data are shown as the mean ± S.D. (n = 4 independent experiments) (exact P value: B-TTPy vs. BD P = 1.00742E-07; CBD vs. MEANING P = 5.00642E-08). Data are analyzed with one-way ANOVA. e Confocal laser scanning microscopy imaging and f quantitative analysis of green fluorescence intensity from calcein acetoxymethyl ester (Calcein AM) in QL01#GBM cells. Scale bar, 10 μm. Data are shown as the mean ± S.D. (n = 4 independent experiments) (exact P value: BD vs. CBD P = 1.89336E-09). Data are analyzed with one-way ANOVA. g Pearson’s correlation coefficient of SSBP1 with Mitotracker Green. Data are shown as the mean ± S.D. (n = 9 images from three independent experiments). Data are analyzed with two-tailed, unpaired Student’s t-test. h HMGB1 release from QL01#GBM cells after different treatments. Data are shown as the mean ± S.D. (n = 3 independent experiments). Data are analyzed with one-way ANOVA. i The levels of ATP release after different treatments. Data are shown as the mean ± S.D. (n = 3 independent experiments) (exact P value: B-TTPy vs. BD P = 6.31757E-05; CBD vs. MEANING P = 4.21364E-05). Data are analyzed with one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. j CXCL10 expression level in QL01#GBM cells after different treatments. Data are shown as the mean ± S.D. (n = 3 independent experiments). Data are analyzed with one-way ANOVA. k IFN-β release from QL01#GBM cells after different treatments. Data are shown as the mean ± S.D. (n = 3 independent experiments). Data are analyzed with one-way ANOVA. Source data are provided as a Source Data file.
