Fig. 2: tr-TILs retain memory status and express exhaustion markers.

a–c Representative proliferation kinetics of (a) expanded tr-TILs divided into high (n = 3), and (b) low-moderate recovery (n = 3), and (c) not expanded (n = 3). d, e Scatter plot with bar graphs showing duration (d) and dosage (e) of steroid treatment with dexamethasone (dex) between expanded tr-TILs-patients (n = 62) vs. not expanded tr-TILs-patients (n = 53). Data are presented as mean ± SD, and statistical significance was assessed using a two-tailed Mann–Whitney test. f, g Scatter plots with bars showing (f) central (Tcm) and effector (Tem) memory status of CD8⁺ and CD4⁺ TILs of expanded (n = 71) and not expanded (n = 74) samples at the end of expansion process (statistical significance was assessed using a two-tailed unpaired t test); (g) and exhaustion immunophenotype on CD8⁺, CD4⁺ tr-TILs for both expanded (n = 72) and not expanded (n = 75) tr-TILs at the end of expansion process. Data are presented as mean ± SD, and statistical significance was assessed using a two-tailed Mann–Whitney test. h–j Real-time PCR relative expression of genes related to (h) progenitor and (i) intermediate exhaustion status from Day 7 to Day 18 in expanded tr-TIL samples (n = 5 and 7 respectively, in blue in the supplementary tables), or related to (j) effector status in not expanded samples from Day 7 to Day 14 (n = 6, marked with an * in the supplementary tables). Data are presented as mean ± SD, with 2 technical replicates averaged prior to statistical analysis assessed using a two-tailed paired t-test. Full numerical data and comprehensive details of statistical analyses are available in the corresponding source data tables for each figure. Source Data are provided as a Source Data file.