Fig. 3: Expanded tr-TILs preserve tumor-specific T cell clonotypes and maintain progenitor status. | Nature Communications

Fig. 3: Expanded tr-TILs preserve tumor-specific T cell clonotypes and maintain progenitor status.

From: Polyclonal expansion of functional tumor-reactive lymphocytes infiltrating glioblastoma for personalized cell therapy

Fig. 3

a Entire tissue sections (upper panel- scale bar, 5 mm) and selected region (lower panel- scale bar, 900 μm) of a representative glioblastoma stained for CD8 (red) and CD137 (brown). b, c Two distinct areas showing CD8+ TILs co-expressing CD137 (scale bars, 200 μm and 50 μm), and (d, e) coexistence of CD8+ CD137+ TILs and CD8+ TILs lacking CD137 (scale bar, 50 μm). f, g Representative immunofluorescence images showing DAPI (blue), CD8 (red) and TCF1 (green) staining and CD8/TCF1 colocalization (merge) of (f) TIL 390 (scale bar, 50 μm) and (g) TIL 431 (scale bar, 50 μm). Immunohistochemistry and immunofluorescence staining were performed on a sample size of n = 10. h Distribution of TCRβ clonotypes by tr-TIL samples from 10 glioma patients. TCRβ chain frequencies are expressed as a percentage, with 2% as the lower cut-off value, and are shown in a Stacked Barplot generated using R 4.2.3 (URL: https://www.R-project.org/). The absolute count of TCR clonotypes is indicated above each barplot.

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