Fig. 3: Phylogenetic analysis of plant oxidosqualene cyclases (OSCs).
From: Biosynthesis and bioactivity of anti-inflammatory triterpenoids in Calendula officinalis
A Maximum-likelihood tree of characterised plant OSCs constructed in IQTree using the JTT matrix-based model. Taraxasterol synthases (TXSSs) are highlighted in orange and mixed-amyrin synthases are highlighted in light blue (Asteraceae) and blue (non-Asteraceae) and annotated with chemical structures of their major products. A full tree showing collapsed clades (grey triangles) is provided in Supplementary Fig. 17 and a list of taxa and accession numbers are provided in Supplementary Data 6. Functionally characterised OSCs are shown in bold, those in red are characterised in this paper (Supplementary Fig. 20). An asterisk (*) indicates likely pseudogenes. Filled grey circles indicate bootstrap supports for each node. The scale bar represents the number of substitutions per site. B Species cladogram of Asteraceae and related species with fully sequenced genomes. Filled box indicates presence of a TXSS gene in the genome or transcriptome, unfilled box indicates absence in the genome. ONS = onocerin synthase; CAS = cycloartenol synthase; PTS = poacetapetol synthase; bAS=β-amyrin synthase; MAS = mixed amyrin synthase; TXSS = taraxasterol/Ψ-taraxasterol synthase.
