Fig. 7: Characterisation of pot marigold triterpene acyl transferases, pathway reconstruction and expression analysis.
From: Biosynthesis and bioactivity of anti-inflammatory triterpenoids in Calendula officinalis
A Total ion chromatograms of extracts of N. benthamiana leaves transiently co-expressing CoTXSS with CoACT1-3 or CoTXSS, CoCYP716A392 and CoACT1-3. B Peak area analysis showing products of pathway reconstruction in N. benthamiana through agroinfiltration of indicated genes, quantified compared to friedelin internal standard. Error bars indicate the mean and standard error of 6 biological replicates (independent infiltrations). Statistical significance was inferred using a Kruskal-Wallis test with a post-hoc Dunns test and Benjamini-Hochberg correction, except faradiol palmitate which was inferred by one-way ANOVA and post-hoc Dunnetts test (Supplementary Data 10). C Pathway schematic. P values are provided in Supplementary Data 10. D Relative expression of CoTXSS, CoCYP716A392, CoCYP716A393, CoACT1, CoACT2 through six stages of floral development (S1-6); Error bars indicate the mean and standard deviation of 3 biological replicates (2 technical replicates per sample).
