Fig. 4: DIMT-1 functions in the germline to regulate lifespan and affects the translation of specific mRNA transcripts.
From: The 18S rRNA methyltransferase DIMT-1 regulates lifespan in the germline later in life
a Auxin-inducible degron (AID) ubiquitous degradation of DIMT-1 protein leads to significant decrease in m6,2A levels in the 18S rRNA subunit as assessed by UHPLC-ms/ms. Statistics represent an unpaired two-tailed t-test p = 0.0286. b Ubiquitous and germline-specific AID-induced DIMT-1 protein degradation causes a lifespan extension, while DIMT-1 depletion in the muscle, intestine, or neurons has no effect on lifespan extension. c, d Tissue-specific knockdown of dimt-1 in the germline increases lifespan to a similar extent as in ubiquitous knockdown, while knockdown of dimt-1 in the muscle, intestine, or neurons has no significant effect on longevity. e dimt-1 knockdown does not extend the lifespan of worms treated with 5-fluorodeoxyuridine (FUdR), a drug that inhibits the proliferation of germline stem cells and the production of intact eggs. Statistics and replicate longevity experiments are presented in Supplementary Table 2. ns not-significant, *p < 0.05, ***p < 0.001, ****p < 0.0001 as calculated by log-rank (Mantel–Cox) statistical test.
