Fig. 6: DIMT-1 regulates lifespan after mid-life.

a Experimental design for the AID-tagged DIMT-1 temporal knock out experiments (y.a. young adults). Red lines indicate when strains were placed on 150 uM auxin and when DIMT-1 is knocked down. b AID-induced depletion of DIMT-1 in the germline extends lifespan when depleted in the previous generation and for the entirety of the assayed generation, starting at birth, or from young adulthood for the remainder of the lifespan but does not extend lifespan when depleted only from birth until young adulthood. c AID-induced depletion of DIMT-1 ubiquitously extends lifespan when depleted in the previous generation and for the entirety of the assayed generation, starting at birth, or from young adulthood for the remainder of the lifespan and causes a less dramatic extension in lifespan when depleted only from birth until young adulthood. d AID-induced depletion of DIMT-1 in the germline extends lifespan to a similar extent when depleted from young adulthood, after reproduction, or starting in mid-life. Statistics and replicate longevity experiments are presented in Supplementary Table 3. ns not-significant, *p < 0.05, ***p < 0.001, ****p < 0.0001 as calculated by log-rank (Mantel–Cox) statistical test.