Fig. 1: General workflow for the fully automated synthesis of modified peptides on the Chemputer.
From: Universal peptide synthesis via solid-phase methods fused with chemputation
Reagents used during SPPS, and the accompanying procedures, are translated into a digital χDL script. A digital graph is then generated with the necessary modules to carry out the synthetic operations, and a physical platform is assembled accordingly. Finally, with the digital and physical requirements met, the platform can then execute the requisite synthetic operations, including SPPS, resin cleavage, ether precipitation, and subsequent desired chemical modifications, in a single automated procedure. Fmoc-AAn-OH = 9-fluorenylmethoxycarbonyl protected amino acids; HATU hexafluorophosphate azabenzotriazole tetramethyl uranium, DIPEA N,N-diisopropylethylamine, TIPS triisopropylsilane, TFA trifluoroacetic acid, DMF N,N-dimethylformamide, DCM dichloromethane.
