Fig. 4: Fully automated chemical synthesis of traditionally difficult peptide sequences.
From: Universal peptide synthesis via solid-phase methods fused with chemputation
Crude RP-HPLC traces (214 nm, 60 min 0–80% MeCN gradient) and corresponding ESI-MS of ACP(65–74) (1), 18A (2), and GHRH(1-29) (3). All peptides were synthesized in full automation, including assembly, cleavage, and ether workup. Relative peak areas were used to determine the efficiency of peptide assembly and were denoted as crude purity. Mass spectra were obtained from the summation of the time scale shown.
