Fig. 5: Cell neighborhood phenotyping by ROSIE.
From: ROSIE: AI generation of multiplex immunofluorescence staining from histopathology images
We identify two cellular neighborhood phenotypes of interest: tumor-infiltrating lymphocytes (TILs) and lymphocyte neighboring epithelial cells (LNEs). TILs are defined as cells that are labeled as lymphocytes (B and T cells) and reside in epithelial tissue (using the graph partitioning algorithm). LNEs are epithelial cells that have at least one lymphocyte as a neighbor. TILs are measured as the raw count per sample, whereas LNEs are measured as the proportion of epithelial cells with lymphocyte neighbors. A We visualize three samples (by median Pearson R) of TILs and LNEs based on ground truth-derived and ROSIE-predicted expressions. B Scatter plots of predicted and ground truth measurements, where each dot represents a sample.
