Fig. 1: High throughput nanopore sequencing of XNAs. | Nature Communications

Fig. 1: High throughput nanopore sequencing of XNAs.

From: Direct high-throughput deconvolution of non-canonical bases via nanopore sequencing and bootstrapped learning

Fig. 1

A Overview of workflow, showing the synthesis of XNA templates containing non-canonical basepairs (details in Supplementary Fig. 2), nanopore sequencing, signal-level analysis and direct deconvolution of canonical and non-canonical bases. B Histogram of read lengths for XNA and control DNA libraries (dotted lines indicate median values). C Histogram of template coverage of template-specific region for XNA and control DNA libraries (dotted lines indicate median values). D Bar chart with read counts as a function of barcode distance (computed as edit distance of sequenced barcode to the closest reference barcode). E Read proportion between XNA and DNA sequences for each template, sorted by XNA proportion. Source data are provided as a Source Data file.

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