Fig. 2: Immune cell infiltration is a common feature across animal models of ALS.
From: The CCL2-CCR2 axis drives neuromuscular denervation in amyotrophic lateral sclerosis
A Immunohistochemical analysis of CD45+ cells infiltrating into the innervation zone (NMJs visualised with α-bungarotoxin, BTX) of gastrocnemius (GC) muscle from late-symptomatic (postnatal day 19) hTDP43Tg/Tg mice (Tg/Tg; n = 4) versus wild-type littermate controls (WT; n = 4). B Immunohistochemical analysis of CD45+ cells infiltrating into the innervation zone (NMJs visualised with α-bungarotoxin, BTX) of GC muscle from late-symptomatic TDP-43A315T mice (postnatal day 90; Tg/0; n = 5) versus WT littermate controls (n = 5). C Immunohistochemical analysis of CD45+ cells infiltrating the innervation zone (NMJs visualised with α-bungarotoxin, BTX) of GC muscle from early-symptomatic TDP-43M337V mice (12 months old; Tg/Tg; n = 3) versus WT littermate controls (n = 3). Data points represent an average from 10 fields of view from 1 mouse. Data is presented as mean ± sem. Scale bars = 40 µm. Data analysis: (A) two-tailed Mann-Whitney test [Shapiro-Wilk normality test, WT group: p = 0.0328, Tg/Tg group: p = 0.6879], p = 0.0286, (B) unpaired, two-tailed t-test, p = 0.0004, (C) unpaired, two-tailed t-test, p = 0.0427. * = p < 0.05; *** = p < 0.001.
