Fig. 3: Degree of macrophage infiltration correlates with vulnerability to NMJ denervation.
From: The CCL2-CCR2 axis drives neuromuscular denervation in amyotrophic lateral sclerosis
A–C Immunohistochemical analysis of CD68+ macrophages in the innervation zone of the gastrocnemius (GC; A), tibialis anterior (TA; B) and extensor digitorum longus (EDL; C) muscle (longitudinal section) of control wild-type (WT) and hTDP-43Tg/Tg mice (postnatal day 19; Tg/Tg). NMJs were visualised with α-bungarotoxin (BTX). Scale bar = 40 µm. D–F Quantitative analysis of CD68+ cells in the innervation zone of GC, TA and EDL muscles. Data is presented as mean ± sem. Data analysis: (D) gastrocnemius: n = 4 mice (WT, Tg/Tg), unpaired, two-tailed t-test, p = 0.0034; (E) tibialis anterior: n = 3 (WT), n = 4 (Tg/Tg), unpaired, two-tailed Mann-Whitney test [Shapiro-Wilk normality test, WT group: p = 0.1555, Tg/Tg group: p = 0.0459]; (F) extensor digitorum longus: n = 3 (WT, Tg/Tg), unpaired, two-tailed t-test. n.s. = non-significant; ** = p < 0.01.
