Fig. 5: Proteomic characterisation of inflammatory responses in hTDP-43^Tg/Tg mice.

A Proteome profiler analysis of 111 inflammatory proteins in gastrocnemius (GC), tibialis anterior (TA) and extensor digitorum longus (EDL) muscles and spinal cord (SC) samples from late symptomatic (postnatal day 19) hTDP-43^Tg/Tg mice. Fold change in protein levels is presented relative to wild-type (WT) littermate controls. In the GC muscle, CCL2, CCL3, CCL4, CCL5 and CCL6 chemokines showed the greatest increase in protein levels (magnified and highlighted in the top right panel). Exact fold changes are available in the Source Data file. B–E Quantitative analysis of CCL2 (B), CCL3 (C), CCL4 (D) and CCL5 (E) proteins in the GC and TA muscles and SC using Legendplex immunoassay. Data is presented as mean ± sem. The Legendplex multiplex immunoassay and statistical analysis was performed with n = 4 mice in the GC, TA and SC of the WT group and TA and SC of the Tg/Tg group. The GC of the Tg/Tg group was quantified in n = 3 mice. Data analysis: In each case (B–E), unpaired, one-tailed t-test was performed for direct comparison of WT vs Tg/Tg groups; (B) gastrocnemius: p = 0.0350; (C) gastrocnemius: p = 0.0174; (C) tibialis anterior: p = 0.0318; (C) spinal cord: p = 0.0083; (D) gastrocnemius: p = 0.0209; (E) gastrocnemius: p = 0.0031; (E) tibialis anterior: p = 0.0456. * = p < 0.05; ** = p < 0.01.