Fig. 6: CCL2 expression in the gastrocnemius (GC) muscle across different disease stages.
From: The CCL2-CCR2 axis drives neuromuscular denervation in amyotrophic lateral sclerosis
A–C CCL2 staining (green) in the GC muscle of wild-type (WT; A) and late symptomatic postnatal day 19 (P19) hTDP-43Tg/Tg (Tg/Tg; B, C) mice. Arrows indicate CCL2+ cells in the close vicinity of neuromuscular junctions (NMJs), visualised with α-bungarotoxin (BTX; magenta). C High magnification image of CCL2+ cells around a single NMJ in the GC muscle of late stage hTDP-43Tg/Tg mice. Innervation is shown through NEFH/SV2a (gray) staining. Asterisks label individual nuclei of CCL2+ cells. 3D-reconstructed version of this image is available as Supplementary movie 1. D–F Quantitative analysis of the ratio of NMJs with perisynaptic CCL2-expressing cells in presymptomatic (D), early symptomatic (E) and late symptomatic (F) mice. Data is presented as mean ± sem. Scale bar represents 50 µm on (A, B) and 10 µm on (C). Data analysis: (D) Presymptomatic stage (postnatal day 7): n = 4 (WT), n = 3 (Tg/Tg), unpaired, two-tailed t-test, p = 0.0466; (E) Early symptomatic stage (postnatal day 15): n = 3 (WT, Tg/Tg), unpaired, two-tailed t-test, p = 0.0088; (F) Late symptomatic stage: n = 4 (WT, Tg/Tg), unpaired, two-tailed t-test, p = 0.0011. * = p < 0.05; ** = p < 0.01.
