Fig. 1: Genome-wide mapping of direct IRX3 target genes in preadipocytes.

Chromatin immunoprecipitation followed by sequencing (ChIP-seq) was performed for IRX3 in preadipocytes isolated from inguinal white adipose tissue (iWAT) one day before (day \(-\)1) and one day after (day 1) induction of differentiation (n \(=\) 2 per condition), and from gonadal WAT (gWAT) on day 1 (n \(=\) 1). a Heatmap showing normalized IRX3 peak intensities across all called peaks in iWAT and gWAT in preadipocytes. b Metaprofile plot of aggregate IRX3 binding across all peaks. c Venn diagram of high-confidence IRX3 peaks (q \( < \) 0.001, \(\ge \,\)10-fold enrichment). See also Supplementary Data 1. d Genomic distribution of filtered IRX3 peaks relative to gene features (top) and nearest transcription start sites (TSS) (bottom). e Top 10 significantly enriched REACTOME pathways among IRX3-bound genes per condition. Full list in Supplementary Data 1. f IRX3 target genes involved in chromatin modification. g IRX3 target genes involved in SUMOylation. h Genome browser tracks of IRX3 occupancy at loci encoding SUMO pathway components. Shaded boxes mark peak locations. i Schematic of known and proposed IRX-family DNA binding motifs: preferred minimal motif in Drosophila (1)^31,32, inverted (2)^31,32,33,34, and predicted in humans (3) (JASPAR, unvalidated³⁵). MEME-ChIP motif analysis under filtered peaks reveals a low-significance match to motif 2. j Most significantly enriched motif identified by MEME-ChIP and its top five TOMTOM matches (top), with associated Panther GO terms for all significant (E \( < \) 0.05) matches (bottom). See also Supplementary Data 2–4. k Second most significant MEME-ChIP motif, top five TOMTOM matches, and corresponding Panther GOs for all matches (E \( < \) 0.05). See also Supplementary Data 2–4. Source data are provided in the Source Data file.