Fig. 9: Inhibition of SUMOylation represses IRX3-KO-dependent osteogenesis.
From: IRX3 controls a SUMOylation-dependent differentiation switch in adipocyte precursor cells
To assess whether SUMOylation mediates the IRX3-KO-dependent adipogenesis, ME3 control and IRX3-KO cells were subjected to osteogenic differentiation in 3D culture for 21 days and treated with either vehicle or 0.5 µM ML-792 throughout the differentiation. a Alkaline phosphatase staining. Brightfield view (left) and quantification (right) shown. Data represent n \(=\) 5 replicate wells from a single experiment. Scale bar, 1 mm. ***padj \( < \) 0.001, DMSO vs. ML-792. ns, not significant; ###padj \( < \) 0.001, control vs. IRX3-KO. b Cells were treated as in (a), except RNA was harvested on day 7 and expression of early and intermediate markers of osteogenesis was measured by qPCR. Data normalized to the reference gene Tbp and shown relative to the control group. Data represent n \(=\) 5 replicate wells from a single experiment. *padj \( < \) 0.05, ns, not significant, DMSO vs. ML-792; ##padj \(=\) 0.002, ###padj \( < \) 0.001, control vs. IRX3-KO; two-way ANOVA with Holm–Sidak correction. Data were square root-transformed before statistical calculations. Bar graphs show means \(\pm \,\)SD. Source data are provided in the Source Data file.
