Table 1 Primer sequences
From: IRX3 controls a SUMOylation-dependent differentiation switch in adipocyte precursor cells
Target gene | Application | Forward primer | Reverse primer |
|---|---|---|---|
Senp1 | ChIP-qPCR | 5’-GGAACCGGAACCTTCTTTAC-3’ | 5’-CTCGCTCTTTGACCTCTTGTC-3’ |
Senp5 | ChIP-qPCR | 5’-GCTCTGGACTACAGTTTAAG-3’ | 5’-TCCAGATGAAGGCTCTAC-3’ |
Senp6 | ChIP-qPCR | 5’-GCTCCCACCTCCATATT-3’ | 5’-CCAAGCTTAGGTCTGAAAC-3’ |
Ranbp2 | ChIP-qPCR | 5’-CGGATTTATTAGGGCACTG-3’ | 5’-AGACGAACTCCCTTTCC-3’ |
Sae1 | ChIP-qPCR | 5’-TCCAAGGACACTCTCAATAC-3’ | 5’-GGGTTTCCTAGTGCTTCA-3’ |
Uba2 | ChIP-qPCR | 5’-CTGAGTCCTGGGATTACTT-3’ | 5’-CCGAGGTCTTTAGAAGGT-3’ |
Ubc9 | ChIP-qPCR | 5’-AAACTCCTCTTCGGGATG-3’ | 5’-TGACCCTTGGGAATCTATG-3’ |
Cbx7 | ChIP-qPCR | 5’-CTCAGGATCTCTAGTCCTATC-3’ | 5’-CATCCTGTTCCTCACTTTC-3’ |
Zmiz1 | ChIP-qPCR | 5’-GGCCCTTTGGAAATAGAATAG-3’ | 5’-AAGGAACTCGGCGTTAG-3’ |
Ctr1 (Adipoq) | ChIP-qPCR | 5’-CTACTTGGCTTCCCAGACCC-3’ | 5’-ACCCAGTCAAGGCCAATAGC-3’ |
Ctr2 (gene desert) | ChIP-qPCR | 5’-TACACATTCAGGGTGGAAGCAA-3’ | 5’-TGTCTCTCAGCACTATCTCCCCAA-3’ |
Pparg2 | qPCR | 5’-TTATAGCTGTCATTATTCTCAGTGGAG-3’ | 5’-GACTCTGGGTGATTCAGCTTG-3’ |
Cebpa | qPCR | 5’-GGTTTAGGGATGTTTGGGTTT-3’ | 5’-GCCCACTTCATTTCATTGGT-3’ |
Fabp4 | qPCR | 5’-ATCACCGCAGACGACAGG-3’ | 5’-TCATAACACATTCCACCACCA-3’ |
Plin2 | qPCR | 5’-CTCCACTCCACTGTCCACCT-3’ | 5’-ACTCCACCCACGAGACATAGA-3’ |
Adipoq | qPCR | 5’-ACTCCTGGAGAGAAGGGAGA-3’ | 5’-CGCTGAGCGATACACATAAGC-3’ |
Lipe (Hsl) | qPCR | 5’-CACAAAGGCTGCTTCTACGG-3’ | 5’-GGAACTGGCGGTCACACT-3’ |
Pnpla2 (Atgl) | qPCR | 5’-CAACGCCACTCACATCTACGG-3’ | 5’-GGACACCTCAATAATGTTGGCAC-3’ |
Cd36 | qPCR | 5’-TTGAAAAGTCTCGGACATTGAG-3’ | 5’-TCAGATCCGAACACAGCGTA-3’ |
Ppara | qPCR | 5’-CGGGAAAGACCAGCAACA-3’ | 5’-GAATCGGACCTCTGCCTCT-3’ |
Ucp1 | qPCR | 5’-GGGCATTCAGAGGCAAATCAG-3’ | 5’-TTTCCGAGAGAGGCAGGTGTTT-3’ |
Rps13 | qPCR | 5’-CAGGTCCGTTTTGTGACTG-3’ | 5’-AGCATCCTTATCCTTTCTGTT-3’ |
Tbp | qPCR | 5’-GGGGAGCTGTGATGTGAAGT-3’ | 5’-CCAGGAAATAATTCTGGCTCA-3’ |
Sp7 | qPCR | 5’-GGCGTCCTCTCTGCTTGAG-3’ | 5’-TTTGTGCCTCCTTTCCCCAG-3’ |
Alpl | qPCR | 5’-TGCCCTGAAACTCCAAAAGC-3’ | 5’-GACGCCCATACCATCTCCC-3’ |
Col1a1 | qPCR | 5’-TCTGACTGGAAGAGCGGAGAG-3’ | 5’-TGAGTAGGGAACACACAGGTC-3’ |
Spp1 | qPCR | 5’-GAGGAAACCAGCCAAGGACT-3’ | 5’-CGGGAGGGAGGAGGCAAT-3’ |
