Fig. 5: Influenza virus infection leads to the sustained presence of populations of epithelial cells expressing high levels of MHCI and MHCII. | Nature Communications

Fig. 5: Influenza virus infection leads to the sustained presence of populations of epithelial cells expressing high levels of MHCI and MHCII.

From: Lung structural cells are altered by influenza virus leading to rapid immune protection following re-challenge

Fig. 5

SpiB-mCherry reporter mice were infected with Influenza A Virus IAV-WSN (100-200PFU) and lungs taken at day 0, 10, or 30 post infection. EpCAM1+ epithelial cell populations were identified by gating on lineage (CD45, CD31, and CD140a) negative, SpiB negative or SpiB+ populations. A shows representative staining of MHCII and MHCI expression by ciliated, club and Sca1 progenitor cells at each timepoint. B Data are not normally distributed, each symbol represents a mouse and the bars show median with interquartile range. Differences between timepoints were tested by a one-way Kruskal–Wallis test followed by a Dunn’s multiple comparisons test and separately between SpiB negative and positive populations by paired one-way Wilcoxon rank test. Data are from two independent experiments at each timepoint with a total of n = 21 naive; n = 10 day 10; and n = 10 day 30 mice. Source data for 5B are provided as a Source Data file.

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