Fig. 6: Epithelial cells from IAV re-challenged mice have less virus than those from primary infected animals.

C57BL/6 mice that were either naive or infected 30 days earlier with influenza A virus (IAV)-WSN, were infected with IAV-X31, their lungs harvested 2 days later, and epithelial cells and fibroblasts examined by flow cytometry. A The percentages and number or EpCAM1+ cells positive for IAV-Nucleoprotein (NP) were examined by flow cytometry, gated as shown in SFig. 7C. B The percentages of each epithelial cell type that were IAV-NP+. C Representative FACS plots of IFN-responsive fibroblasts indicating positive populations, cells are gated on live, single, dump negative (CD45/CD31/EpCAM1) cells that are CD140a+ gated on CD49e+ CD9+ fibroblasts that are Bst2+ as shown in SFig. 9B. D the percentages and numbers of IFN-responsive fibroblasts. Data are from two independent experiments combined. Primary: n = 8, re-infection: n = 9. In all graphs, each symbol represents a mouse, and the bars show means with SEM error bars for normally distributed data and median with interquartile range for non-normally distributed data. A Data are not normally distributed. B All data are not normally distributed apart from club cells. D The percentages and numbers of IFN-responsive fibroblasts are not normally distributed. In all graphs, normally distributed data tested via one-way t test, and non-normally distributed data tested via one-way Mann–Whitney test. Source data for A, B, D are provided as a Source Data file.