Fig. 1: CRISPR screening reveals RING finger E3 ubiquitin/SUMO ligase genes required for WEE1i-tolerance. | Nature Communications

Fig. 1: CRISPR screening reveals RING finger E3 ubiquitin/SUMO ligase genes required for WEE1i-tolerance.

From: The RING finger E3 ligase RNF25 protects DNA replication forks independently of its canonical roles in ubiquitin signaling

Fig. 1

a Experimental design of the CRISPR screens performed in this study. b, c (Top) Results of CRISPR screens showing dropout of sgRNAs in vehicle (DMSO)- and WEE1i-treated Pa02C and Pa03C cells. Log2-transformed sgRNA abundance scores and statistical significance were calculated using Völundr. (Bottom) DrugZ-calculated normZ score is plotted vs. gene rank for WEE1i screen. Synthetic lethality has the lowest negative normZ score, and drug resistance has the highest positive normZ score. d Effect of TRIM11-directed sgRNAs on clonogenic survival of Pa03C cells. e Effect of PIAS-deletion on WEE1-sensitivity in Pa03C cells. Effect of RNF25-deficiency on WEE1-sensitivity in Pa02C (f) and Pa03C (g) cells. All data in (b, c) represent counts from three biological replicates and (d–g) represent mean ± SD from triplicate wells and are representative of two biological replicates. Statistics: b, c two-sided two-sample Kolmogorov–Smirnov test. Source data are provided as a Source Data file.

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