Fig. 2: RNF25 prevents ssDNA accumulation and facilitates S-phase and G2/M progression of WEE1i-treated cells. | Nature Communications

Fig. 2: RNF25 prevents ssDNA accumulation and facilitates S-phase and G2/M progression of WEE1i-treated cells.

From: The RING finger E3 ligase RNF25 protects DNA replication forks independently of its canonical roles in ubiquitin signaling

Fig. 2

a Effect of WEE1i treatment on levels of the indicated DNA damage markers in Pa03C sgNT and sgRNF25 cells. b Effect of WEE1i treatment on cell cycle profiles of Pa03C sgNT and sgRNF25 cells. c Effect of WEE1i treatment on levels of ssDNA in Pa03C sgNT and sgRNF25 cells. d Cell fate maps showing effect of WEE1i-treatment on interphase and mitotic progression of Pa03C sgNT and sgRNF25 cells. e Effect of WEE1i-treatment on multinucleation in Pa03C sgNT and sgRNF25 cells. All data represent mean ± SD from four separate populations of cells per condition. Statistics: Ordinary one-way ANOVA with Tukey’s multiple comparisons test. All flow cytometry data (b, c) represent means from duplicate dishes and are representative of two biological replicates. Source data are provided as a Source Data file.

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