Fig. 7: RNF25 and PolĪ¶ function in a common DNA replication fork protection pathway. | Nature Communications

Fig. 7: RNF25 and PolĪ¶ function in a common DNA replication fork protection pathway.

From: The RING finger E3 ligase RNF25 protects DNA replication forks independently of its canonical roles in ubiquitin signaling

Fig. 7

H1299 WT and RNF25 KO cells were transfected with indicated siRNAs for 48ā€‰h before conducting the DNA fiber fork degradation (a) or fork movement (b) assay. 150 fibers were analyzed per condition with means shown using bars. This Fig. is a representative experiment from two biological replicates. Statistics: two-tailed Mann-Whitney test. c H1299 cells were transfected with indicated siRNAs before HA-RNF25 was expressed using adenoviral vectors and immunoprecipitated with anti-HA beads. d Schematic depicting the role of RNF25 in basal replication and following replication stress. Under basal conditions, RNF25 promotes fork movement along with REV7 and REV3L. Under replication stress conditions, RNF25 protects reversed forks by recruiting REV7. REV1 and REV3L also function in the same fork protection pathway. However, in RNF25 deficient cells, fork degradation is mediated by nucleases MRE11 and CtIP. Source data are provided as a Source Data file.

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