Fig. 3: Degradation of Las17 lowers DSB mobility.
A Functional domains of Las17, Vrp1, Myo3, and Myo5. Arrows indicate known interactions between these proteins. Not to scale. B Western blot analysis of Las17-AID ± auxin (IAA) (1 mM). IAA or an equivalent amount of 100% ethanol was added 1 h before adding galactose. Mouse α-Myc probed for Las17-AID. Mouse α-Pgk1 was probed as a loading control. Source data are provided as a Source Data file. C MSD analysis (∆t = 30 s) of DSB relative to the SPB in FZ015 (n = 11), FZ075 (Las17-AID) (n = 13), and FZ075 with IAA (1 mM) added 2 h after galactose (Las17-AID + IAA) (n = 13), 3 h after Gal-HO induction. Imaging and analysis done as described in Fig. 1C. Mean ± SEM is shown. Source data are provided as a Source Data file. D Rc from MSD analysis of strains in (C). Statistical analysis for the radius of confinement derived in PRISM using a one-way Anova (ns p ≥ 0.05, *p < 0.05, **p < 0.01, and ***p < 0.001). WT vs. Las17-AID (p = 0.9381), WT vs. Las17-AID + IAA (p = 0.0211), and Las17-AID vs. Las17-AID + IAA (p = 0.006). See Supplementary Data 1, 2 for significance and RC values. Mean ± SEM is shown. Source data are provided as a Source Data file. E MSD analysis (∆t = 30 s) of DSB relative to the SPB in FZ015 (n = 22), FZ099 (las17-CA∆) (n = 12), FZ219 (las17-WH2∆) (n = 10), and FZ100 (las17-WH2-CA∆) (n = 15) 3 h after Gal-HO induction. Imaging and analysis done as described in Fig. 1C. Mean ± SEM is shown. Source data are provided as a Source Data file. F RC from MSD analysis of strains in (E). Statistical analysis for the radius of confinement derived in PRISM using a one-way Anova (ns p ≥ 0.05, *p < 0.05, **p < 0.01, and ***p < 0.001). WT vs. las17-WH2-CAΔ (p = 0.0002), WT vs. las17-WH2∆ (p = 0.0004), and WT vs. las17-CAΔ (p = 0.9693). See Supplementary Data 1, 2 for significance and RC values. Mean ± SEM is shown. Source data are provided as a Source Data file.
