Fig. 4: The role of Myo3 and Myo5 in DSB mobility.
A MSD analysis (∆t = 30 s) of DSB relative to the SPB in FZ015 (WT) (n = 21), FZ033 (myo3∆) (n = 16), and FZ034 (myo5∆) (n = 18) 3 h after Gal-HO induction. Imaging and analysis done as described in Fig. 1C. Mean ± SEM is shown. Source data are provided as a Source Data file. B MSD analysis (∆t = 30 s) of basal mobility of the MAT locus relative to the SPB in FZ152 (WT) (n = 11), FZ215 (myo5∆) (n = 11), and FZ216 (myo3∆) (n = 11). Imaging and analysis done as described in Fig. 1C. Mean ± SEM is shown. Source data are provided as a Source Data file. C RC from MSD analysis of strains in (B). Statistical analysis for the radius of confinement derived in PRISM using a one-way Anova (ns p ≥ 0.05, *p < 0.05, **p < 0.01, and ***p < 0.001). WT vs. myo5Δ (p = 0.9782) and WT vs. myo3Δ (p = 0.8783). See Supplementary Data 1, 2 for significance and RC values. Mean ± SEM is shown. Source data are provided as a Source Data file. D MSD analysis (∆t = 30 s) of the DSB in FZ015 (WT) (n = 21), FZ033 (myo3∆) (n = 16), and FZ047 (2 copies of Myo5 myo3∆) (n = 15) 3 h after Gal-HO induction. Imaging and analysis done as described in Fig. 1C. Mean ± SEM is shown. Source data are provided as a Source Data file. E MSD analysis (∆t = 30 s) of the DSB in FZ015 (WT) (n = 12), FZ034 (myo5∆) (n = 18), and FZ220 (2 copies of Myo3 myo5∆) (n = 10) 3 h after Gal-HO induction. Imaging and analysis done as described in Fig. 1C. Mean ± SEM is shown. Source data are provided as a Source Data file. F Rc from MSD analysis of strains in (A), (D), and (E). Statistical analysis for the radius of confinement derived in PRISM using a one-way Anova (ns p ≥ 0.05, *p < 0.05, **p < 0.01, and ***p < 0.001). WT vs. myo5Δ (p = 0.0048), WT vs. myo3Δ (p = 0.0033), WT vs. myo3Δ, second copy of Myo5 (p = 0.9933), and WT vs. myo5Δ, second copy of Myo3 (p = 0.9998). See Supplementary Data 1, 2 for significance and RC values. Mean ± SEM is shown. Source data are provided as a Source Data file. G MSD analysis (∆t = 30 s) of DSB relative to the SPB in FZ015 (WT) (n = 24), FZ074 (myo3∆ Myo5-AID) (n = 21), and FZ074 (myo3∆ Myo5-AID) + IAA (1 mM) (n = 19) 3 h after Gal-HO induction. Imaging and analysis done as described in Fig. 1C. Mean ± SEM is shown. Source data are provided as a Source Data file. H Rc from MSD analysis of strains in (G). Statistical analysis for the radius of confinement derived in PRISM using a one-way Anova (ns p ≥ 0.05, *p < 0.05, **p < 0.01, and ***p < 0.001). WT vs. myo3Δ, Myo5-AID (p = 0.0202), myo3Δ, Myo5-AID vs. myo3Δ, Myo5-AID + IAA (p = 0.0234), and WT vs. myo3Δ, Myo5-AID + IAA (p = 0.939). See Supplementary Data 1, 2 for significance and RC values. Mean ± SEM is shown. Source data are provided as a Source Data file.
