Fig. 5: Arp2/3 is required to initiate resection. | Nature Communications

Fig. 5: Arp2/3 is required to initiate resection.

From: Arp2/3 and type-I myosins control chromosome mobility and end-resection at double-strand breaks in S. cerevisiae

Fig. 5

A qPCR-based resection assay. Pairs of primers are placed 10 kb, 5 kb, and 0.7 kb upstream of the HO-cut site. Between each set of primers is a StyI cleavage site. StyI is unable to cut ssDNA. Resection analysis was done from a minimum of 3 biological replicates collected in triplicate. See “Methods” section for more details. B Resection analysis of FZ015 (WT) and FZ075 (Las17-AID). IAA was added either 1 h before galactose or 2 h after galactose. Samples were collected at 0, 1, 3, and 6 h after adding galactose. Mean ± SEM is shown. Source data are provided as a Source Data file. C Resection analysis of FZ015 (WT), FZ033 (myo3∆), and FZ034 (myo5∆). IAA was added either 1 h before galactose or 2 h after galactose. Samples were collected at 0, 1, 3, and 6 h after adding galactose. Mean ± SEM is shown. Source data are provided as a Source Data file. D Resection analysis of FZ015 (WT) and FZ074 (myo3∆ Myo5-AID). IAA was added either 1 h before galactose or 1 h after galactose. Samples were collected at 0, 1, 2, 3, 4, 5, 6 h after adding galactose. Mean ± SEM is shown. Source data are provided as a Source Data file.

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