Fig. 6: Changes in the rate of resection lower DSB mobility.
A Resection analysis of FZ015 (WT) and FZ151 (fun30∆). Samples were collected at 0, 1, 3, and 6 h after adding galactose. Resection analysis was done from a minimum of 3 biological replicates collected in triplicate. See “Methods” section for more details. Mean ± SEM is shown. Source data are provided as a Source Data file. B MSD analysis (∆t = 30 s) of DSB relative to the SPB in FZ015 (WT) (n = 12) and FZ151 (fun30∆) (n = 12), 3 h after Gal-HO induction. Imaging and analysis done as described in Fig. 1C. Mean ± SEM is shown. Source data are provided as a Source Data file. C RC from MSD measurements in (B). Statistical analysis for the radius of confinement derived in PRISM using a two-sided unpaired t-test (ns p ≥ 0.05, *p < 0.05, **p < 0.01, and ***p < 0.001). WT vs. fun30Δ (p = 0.246). See Supplementary Data 1 and 2 for significance and RC values. Mean ± SEM is shown. Source data are provided as a Source Data file. D MSD analysis (∆t = 30 s) of DSB relative to the SPB in FZ015 (WT) (n = 13), FZ159 (exo1∆ DNA2-AID) (n = 11), and FZ159 (exo1∆ DNA2-AID) + IAA (n = 12) 3 h after Gal-HO induction. IAA (1 mM) was added 1 h before image collection. Imaging and analysis done as described in Fig. 1C. Mean ± SEM is shown. Source data are provided as a Source Data file. E RC from MSD analysis of strains in (D). Statistical analysis for the radius of confinement derived in PRISM using a one-way Anova (ns p ≥ 0.05, *p < 0.05, **p < 0.01, and ***p < 0.001). WT vs. exo1Δ DNA2-AID (p = 0.999), WT vs. exo1Δ DNA2-AID + IAA (p = 0.0266), and exo1Δ DNA2-AID vs. exo1Δ DNA2-AID + IAA (p = 0.0308). See Supplementary Data 1 and 2 for significance and RC values. Mean ± SEM is shown. Source data are provided as a Source Data file.
