Fig. 1: Lack of MacroD1 decreases oxidative metabolism in mouse muscle.

A Weight gain of MacroD1^−/− (KO) and wildtype (WT) male (♂) and female (♀) mice on a standard chow diet was monitored weekly over the course of 2 months. Respective weight measures at week 2 are shown as an example. B Fat mass of female (♀) and male (♂) MacroD1^−/− (KO) and wildtype (WT) animals. Data from n = 6–8 individual mice is shown with the line indicating the median. Statistical analyses were performed using a two-tailed student t-test (*, p < 0.05; **, p < 0.005; ***, p < 0.0005). C–E Age-matched male MacroD1^−/− (KO) and wildtype (WT) mice were submitted to involuntary running until exhaustion, and total running time (C), respiratory exchange ratio (RER) (D), and oxygen consumption (E) were measured. Data is shown as mean ± SD from n = 6–8 individual mice. For statistical analysis, a two-way Anova with Geisser-Greenhouse correction as performed (*, p < 0.05; **, p < 0.005; ***, p < 0.0005) on data from n = 6–8 individual mice. F Gene expression analysis on muscle lysates from MacroD1^−/− and wildtype mice. Data is shown as mean ± SD from n = 5 individual mice. Statistical analyses were performed using a two-tailed student t-test (*, p < 0.05; **, p < 0.005; ***, p < 0.0005). Source data are provided as a Source Data file.