Fig. 6: METTL9 interacts with secretory pathway-related proteins in mNSCs.
From: METTL9 sustains vertebrate neural development primarily via non-catalytic functions
a Volcano plot showing the proteins enriched upon METTL-IP (anti-FLAG) over the control-IP (IgG) in Mettl9Deg NSCs (q value < 0.01); on the right, zoom-in on the enriched interactors, among which the known METTL9-interactors Faf2 and Canx (in black, italics). Y axis represents the -log10 p value after Benjamini-Hochberg multiple test correction (one-sided moderated t-statistics; P[X > x]). b Network showing the genes belonging to the top GO terms enriched in METTL9 interactors relative to the secretory pathway. c, d WB showing the immunoprecipitation of STMN1-HA (IP) (c) or HA-RAB2a (d) with anti-HA beads (HA) or IgG (ctrl), after co-expression of STMN1-HA or HA-RAB2a and METTL9-FLAG in WT mESCs. WB signal: anti-FLAG and anti-HA. N = 2 co-IP (and differentiation) experiments, run in N = 4 WB. e AlphaFold modelling prediction of STMN1-METTL9 protein complexes (STMN1, in orange; METTL9 in green). f Bar graph showing in vitro METTL9 methyltransferase activity (MTase) of recombinant GST-METTL9-FLAG (GST-METTL9) protein with the SLC30A7163-180 peptide or with GST-STMN1-HA (GST-STMN1) and GST (first, second and third bar, respectively); GST-METTL9 activity was also measured with increasing concentrations (μM) of GST or GST-STMN1. The correlation between MTase activity and concentration of GST or GST-STMN1 is expressed by the R2 and p values (linear regression). Error bars represent mean + SE of N = 3 independent experiments. A.U. is arbitrary unit.
