Fig. 1: Metabolic health outcomes of 3xTg-AD mice on different feeding regimens.

A Experimental design: Six-month-old female and male 3xTg-AD and NTg mice were placed on either ad libitum (AL), Diluted AL (DL), or Caloric Restricted (CR, with fasting between once-daily meals) diet and phenotyped over the course of the next 9 months. B–F n = 8 AL, n = 7 DL and n = 7 CR fed 3xTg biologically independent female mice. G–K n = 8 AL, n = 5 DL, n = 7 CR 3xTg biologically independent male mice. B, G, F, K Two-way RM ANOVA followed by Dunnett’s test vs. AL. *p < 0.05 AL vs DL, # p < 0.05 AL vs CR. C–E, H–J Statistics for the overall effect of diet (D), time (T), and the interaction (DxT) represent the p values from a two-way ANOVA; *p < 0.05 from a two-tailed Sidak’s post-test examining the effect of parameters identified as significant in the two-way ANOVA. The overall effect of sex (S) represents the p value from a separately completed three-way ANOVA. B AL vs DL *p = 0.003, AL vs CR #p = 0.0100 (3 months), AL vs DL *p = 0.0015, AL vs CR #p = 0.0022 (6 months), AL vs DL *p < 0.0001, AL vs CR #p = 0.0001 (9 months). C AL vs DL *p < 0.0001, AL vs CR *p < 0.0001. D AL vs DL *p = 0.0015, AL vs CR *p = 0.0004 (E) Al vs DL *p = 0.0002, AL vs CR *p < 0.0001 (F) AL vs DL *p = 0.0002, AL vs CR #p = 0.0005 (3 months), AL vs DL *p = 0.0005, AL vs CR #p = 0.0008 (6 months) AL vs DL *p = 0.0003, AL vs CR #p = 0.0005 (9 months). G AL vs DL *p = 0.0398, AL vs CR #p = 0.0493 (6 months), AL vs DL *p = 0.0148 (9 months) (H) AL vs DL *p = 0.0048, DL vs CR *p = 0.0237 (I) AL vs DL *p = 0.0450 (K) AL vs DL *p < 0.0001, AL vs CR #p < 0.0001(3 months), AL vs DL *p = 0.0030, AL vs CR #p = 0.0005 (6 months), AL vs DL *p = 0.0003, AL vs CR #p = 0.0007 (9 months). Data represented as mean ± SEM. FC: Food Consumption. Source data are provided as a Source Data file.